Fig 1: miR-20a-5p downregulates SHOC2 in 5-FU resistant CCA cells via direct binding. (A) The potential target gene of miR-20a-5p predicted via online tools (ENCORI, Target Scan, miRDB, and MirTarBase) and drawn by Venny online analysis software; (B) The predicted binding site of SHOC2 and miR-20a-5p; (C) The mRNA expression of SHOC2 was negatively correlated with the miR-20a-5p in CCA tissues; (D, E) upregulation of miR-20a-5p by its mimic decreased the expression of SHOC2 both in mRNA (D) and protein levels (E) of QBC939-R and Huh-28-R cells.
Fig 2: FALEC increased the 5-FU resistance of CCA cells by miR-20a-5p/SHOC2 axis. (A, B) Overexpression of FALEC (FALEC-OE) in normally cultured QBC939 and Huh-28 cells increased their resistance to 5-FU significantly in both cells; *, p<0.05, **, p<0.01, compared with blank control; #, p<0.05, ##, p<0.01, compared with FALEC-OE; (C, D) protein expression of SHOC2 and its downstream activator protein pERK1/2 increased after enhancing FALEC expression (FALEC-OE) in both cell lines. Upregulation of miR-20a-5p or knockdown SHOC2 (si-SHOC2) abolished the enhancing effect to SHOC2, and pERK1/2 caused by FALEC.
Supplier Page from Abcam for Anti-Shoc2 / Sur8 antibody